Superiorly, the greater wing of the sphenoid bone at the level of the foramina ovale and spinosum borders the fossa, while inferiorly, there is no bony anatomical structure to limit it, just the medial pterygoid muscle as its runs anteriorly to attach to the mandible. The maxilla forms the anterior border of the cavity and the styloid and condylar processes make up the posterior barricade. Medially, the sphenoid and the palatine bone form a vertical bony rest and laterally the ramus and coronoid process cover the fossa's opening.
The following anatomical structures can be found to pass through the infratemporal fossa and are not strictly limited to the area or part of its supply if deemed to be a nerve or vessel. The temporalis muscle, the lateral pterygoid muscle and the medial pterygoid muscle, the maxillary artery, the pterygoid venous plexus, the mandibular nerve, the posterior superior alveolar nerve, the chorda tympani, the otic ganglion and the lesser petrosal nerve.
The vascular supply of the infratemporal fossa comes singularly from the maxillary artery. The branches have been divided into three sections for clarity and are categorized according to the anatomical structure closest to them, including the mandibular part, the pterygoid part and the pterygopalatine part.
The first part which is the mandibular part contains the arterial branches including the deep auricular, the anterior tympanic, the middle and accessory meningeal arteries and the inferior alveolar artery. The branches pass between the mandible and the sphenomandibular ligament. The second part otherwise known as the pterygoid part contains vessels that pass obliquely and anterosuperiorly between the mandibular ramus and the temporalis muscle. The list of vascular constituents includes the anterior and posterior deep temporal artery, the masseteric artery, the pterygoid artery and the buccal artery.
Lastly, the third part is that which holds the posterior superior alveolar artery that runs from the infratemporal fossa to the pterygopalatine fossa via the pterygomaxillary fissure.
Venous drainage is managed solely by the pterygoid plexus which is most dense in and around the infratemporal fossa.
The infratemporal fossa is densely innervated due to the large amount of musculature and vessels that pass through it and that are situated adjacently. The maxillary nerve and the mandibular nerve contribute their branches, with the mandibular nerve providing nerves from both its anterior and posterior divisions. The anterior division contains the masseteric nerve, the anterior and posterior deep temporal, the medial and lateral pterygoid nerves and the buccal nerve. Meanwhile, the posterior division includes the auriculotemporal nerve, the lingual nerve, the inferior alveolar nerve and the mylohyoid nerve. The maxillary nerve gives a single branch known as the posterior superior alveolar nerve. Other nervous structures are mentioned above in the contents of the fossa save the parasympathetics of the parotid gland, which can be found deep in the most medial part of the fossa.