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The testes or testicles are part of the male genitalia or sexual reproductive organ. It is a structure that is ovoid in shape and between four to six centimeters in length. These bilateral though not always symmetrical anatomical structures are the site of sperm production.



The testis are retroperitoneal from the time of development, until they descend into their final adult position in the scrotum. The testicular tissue is comprised of two hundred to three hundred lobules that are separated by connective tissue septa.

Testis - histological slide

Each lobule contains between two and three seminiferous tubules that are coiled extremely tight and are approximately one meter in length when straight. It is here that the spermatozoa and sex hormones are produced. The tubules converge in the posterior region of the testis and the collective amount of product is released into the network of the rete testis.

Seminiferous tubules - histological slide



The testes hang in suspension attached to the spermatic cord and are covered externally by the loose, wrinkly, pigmented skin known as the scrotum.

Spermatic cord - ventral view

Just inside the scrotum, there are three more distinguishable layers that protect the testicular tissue. The outermost layer is known as the tunica albuginea and is a tough fibrous capsule. The middle and innermost layers are actually one layer known as tunica vaginalis which is split into a visceral layer and a parietal layer. They are derived from the peritoneal outpocketing that occurs during embryonic development. The layers are separated by a serous fluid that allows testes to move in the scrotal sac.

Tunica vaginalis - histological slide

A posterior deficiency is apparent for the transmission of the epididymis and the blood vessels. The visceral layer is in the middle and it is a serous layer that covers the testes, epididymis and the distal part of the spermatic cord. The innermost layer is the parietal tunica vaginalis.

Blood Supply & Lymphatics

The testicular artery is the main form of blood flow to the area and is drained through the pampiniform plexus. The lymph vessels ascend with the testicular vessels and drain into the lumbar nodes.

Testicular artery - ventral view


Cryptorchidism is a phenomenon based on a developmental malfunction that occurs when the testes fail to descend from their embryonic position inside the abdominal cavity, into the scrotum. Even after surgical correction, this disorder is quite severe, seeing as it has been proven to be associated with testicular atrophy, sterility and a much higher risk of developing various germ cell tumors, in particular seminoma and embryonal carcinoma.

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Show references


  • Kyung Won Chung and Harold M. Chung, Board Review Series Gross Anatomy, 6th Edition, Wolters Kluwer - Lippincott, Williams and Wilkins, Chapter 6 Perineum and Pelvis, IV Male Genital Organs, A. Testes, Page 439.
  • Frank H. Netter, MD, Atlas of Human Anatomy, Fifth Edition, Saunders - Elsevier, Chapter 5 Pelvis & Perineum, Subchapter 39. Testes, Epididymis and Ductus Deferens, Guide Pelvis & Perineum: Testes, Epididymis and Ductus Deferens, Testes, Page 195.
  • Arthur S. Schneider and Philip A. Szanto, Board Review Series Pathology, 1st Edition, Wolters Kluwer - Lippincott, Williams and Wilkins, Chapter 18, Male Reproductive System, Page 277.


  • Dr. Alexandra Sieroslawska


  • Testis - Begoña Rodriguez
  • Testis - histological slide - Smart In Media
  • Seminiferous tubules - histological slide - Smart In Media
  • Spermatic cord - ventral view - Hannah Ely
  • Tunica vaginalis - histological slide - Smart In Media
  • Testicular artery - ventral view - Hannah Ely
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